Personal tools
You are here: Home Publikationen Metagenomics to paleogenomics: large-scale sequencing of mammoth DNA.
Navigation
 
Document Actions

Hendrik N Poinar, Carsten Schwarz, Ji Qi, Beth Shapiro, Ross D Macphee, Bernard Buigues, Alexei Tikhonov, Daniel H Huson, Lynn P Tomsho, Alexander Auch, Markus Rampp, Webb Miller, and Stephan C Schuster (2006)

Metagenomics to paleogenomics: large-scale sequencing of mammoth DNA.

Science, 311(5759):392-394.

We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.

 

Powered by Plone CMS, the Open Source Content Management System